About 90.3% of nurses reported to have experienced the sharp injuries and about 37.6% of nurses occurred more than one time sharp injuries by opening the glass ampoules during recent 6 months. The sharp, jagged edges formed when the ampoule neck breaks incompletely pose a serious danger to medical professionals. Fingers and hands can be cut from the sharp jagged edge formed where the neck breaks. Ampoule related injuries are frequent and dangerous Thus, present study developed the new ampoule opener by systematic innovation of user-centered design to avoid painful and dangerous sharps injuries. User needs analysis have been conducted from district hospital, regional hospital and medical center medical center. A questionnaire was used to survey the behaviors (tasks of opening ampoule, types of ampoules, user requirements etc.) and sharp injuries while opening glass ampoules. After analyzing patents search, user needs analysis and results of questionnaire survey, present study designed and built the prototype of ampoule opener. Results of ergonomic evaluation showed that applied the ampoule opener had lower EMG activity of forearm and lower ulnar deviation on wrist. This easy-to-use multi-use ampoule opener avoids the unacceptable sharps injury rates seen when ampoules are opened by hand. The findings of this study could provide references to medical personnel in order to prevent and decrease sharp injuries as much as possible. In clinical effectiveness will be further assessed.